gmp r d systems Search Results


gmp r d systems  (R&D Systems)
94
R&D Systems gmp r d systems
Gmp R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gmp r d systems - by Bioz Stars, 2026-02
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1 il 3  (R&D Systems)
93
R&D Systems 1 il 3
1 Il 3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
1 il 3 - by Bioz Stars, 2026-02
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cyclic gmp  (R&D Systems)
90
R&D Systems cyclic gmp
Cyclic Gmp, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
cyclic gmp - by Bioz Stars, 2026-02
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gmp  (R&D Systems)
92
R&D Systems gmp
Gmp, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
gmp - by Bioz Stars, 2026-02
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human rh gm csf  (R&D Systems)
92
R&D Systems human rh gm csf
Human Rh Gm Csf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
human rh gm csf - by Bioz Stars, 2026-02
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flt 3 ligand  (R&D Systems)
93
R&D Systems flt 3 ligand
Flt 3 Ligand, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
flt 3 ligand - by Bioz Stars, 2026-02
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recombinant human sonic hedgehog shh c24ii n term gmp  (R&D Systems)
93
R&D Systems recombinant human sonic hedgehog shh c24ii n term gmp
Recombinant Human Sonic Hedgehog Shh C24ii N Term Gmp, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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tnfα  (R&D Systems)
94
R&D Systems tnfα
SM-164 induces apoptosis of breast cancer cells in combination with <t>TNFα</t> released by tumor-associated macrophages. ( A ) The <t>parental</t> <t>MDA-MB-231</t> cells were treated with vehicle, TNFα (1 ng/ml) or TNFα + the indicated doses of SM-164 or BV6 overnight. Annexin V + PI +/− apoptotic cells were analyzed by flow cytometry. ( B ) Parental MDA-MB-231 cells were treated with the indicated doses of SM-164, BV6 or AT-406 alone, or with a combination of them plus TNFα (1 ng/ml) for 8 hours. Cell lysates were used to test protein levels of cIAP1/cIAP2 and GAPDH. ( C ) 1 × 10 4 GFP + MDA-MB-231 cells were cultured alone or together with WT mouse BM cells in the presence of M-CSF +/− PBS (P) or IL-4 for 3 d, during which 3 nM SM-164 and 1 μg/ml TNFR:Fc (R:Fc) were added for the last 16 hr (last group on right +R:Fc). Annexin V + PI +/− apoptotic cells in the GFP + population were analyzed by flow cytometry (left panel). The total number of GFP + cells (left graph) was calculated based on % of GFP + cells in the total cell number, and % of Annexin V + PI +/− apoptotic cells in the GFP + population (right graph) was calculated. *p < 0.05 and **p < 0.01, one-way ANOVA +/Dunnett test.
Tnfα, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tnfα/product/R&D Systems
Average 94 stars, based on 1 article reviews
tnfα - by Bioz Stars, 2026-02
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5036 gmp  (R&D Systems)
92
R&D Systems 5036 gmp
SM-164 induces apoptosis of breast cancer cells in combination with <t>TNFα</t> released by tumor-associated macrophages. ( A ) The <t>parental</t> <t>MDA-MB-231</t> cells were treated with vehicle, TNFα (1 ng/ml) or TNFα + the indicated doses of SM-164 or BV6 overnight. Annexin V + PI +/− apoptotic cells were analyzed by flow cytometry. ( B ) Parental MDA-MB-231 cells were treated with the indicated doses of SM-164, BV6 or AT-406 alone, or with a combination of them plus TNFα (1 ng/ml) for 8 hours. Cell lysates were used to test protein levels of cIAP1/cIAP2 and GAPDH. ( C ) 1 × 10 4 GFP + MDA-MB-231 cells were cultured alone or together with WT mouse BM cells in the presence of M-CSF +/− PBS (P) or IL-4 for 3 d, during which 3 nM SM-164 and 1 μg/ml TNFR:Fc (R:Fc) were added for the last 16 hr (last group on right +R:Fc). Annexin V + PI +/− apoptotic cells in the GFP + population were analyzed by flow cytometry (left panel). The total number of GFP + cells (left graph) was calculated based on % of GFP + cells in the total cell number, and % of Annexin V + PI +/− apoptotic cells in the GFP + population (right graph) was calculated. *p < 0.05 and **p < 0.01, one-way ANOVA +/Dunnett test.
5036 Gmp, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/5036 gmp/product/R&D Systems
Average 92 stars, based on 1 article reviews
5036 gmp - by Bioz Stars, 2026-02
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220 gmp  (R&D Systems)
94
R&D Systems 220 gmp
SM-164 induces apoptosis of breast cancer cells in combination with <t>TNFα</t> released by tumor-associated macrophages. ( A ) The <t>parental</t> <t>MDA-MB-231</t> cells were treated with vehicle, TNFα (1 ng/ml) or TNFα + the indicated doses of SM-164 or BV6 overnight. Annexin V + PI +/− apoptotic cells were analyzed by flow cytometry. ( B ) Parental MDA-MB-231 cells were treated with the indicated doses of SM-164, BV6 or AT-406 alone, or with a combination of them plus TNFα (1 ng/ml) for 8 hours. Cell lysates were used to test protein levels of cIAP1/cIAP2 and GAPDH. ( C ) 1 × 10 4 GFP + MDA-MB-231 cells were cultured alone or together with WT mouse BM cells in the presence of M-CSF +/− PBS (P) or IL-4 for 3 d, during which 3 nM SM-164 and 1 μg/ml TNFR:Fc (R:Fc) were added for the last 16 hr (last group on right +R:Fc). Annexin V + PI +/− apoptotic cells in the GFP + population were analyzed by flow cytometry (left panel). The total number of GFP + cells (left graph) was calculated based on % of GFP + cells in the total cell number, and % of Annexin V + PI +/− apoptotic cells in the GFP + population (right graph) was calculated. *p < 0.05 and **p < 0.01, one-way ANOVA +/Dunnett test.
220 Gmp, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/220 gmp/product/R&D Systems
Average 94 stars, based on 1 article reviews
220 gmp - by Bioz Stars, 2026-02
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100 ng/ml gmp-compliant recombinant human m-csf  (R&D Systems)
90
R&D Systems 100 ng/ml gmp-compliant recombinant human m-csf
SM-164 induces apoptosis of breast cancer cells in combination with <t>TNFα</t> released by tumor-associated macrophages. ( A ) The <t>parental</t> <t>MDA-MB-231</t> cells were treated with vehicle, TNFα (1 ng/ml) or TNFα + the indicated doses of SM-164 or BV6 overnight. Annexin V + PI +/− apoptotic cells were analyzed by flow cytometry. ( B ) Parental MDA-MB-231 cells were treated with the indicated doses of SM-164, BV6 or AT-406 alone, or with a combination of them plus TNFα (1 ng/ml) for 8 hours. Cell lysates were used to test protein levels of cIAP1/cIAP2 and GAPDH. ( C ) 1 × 10 4 GFP + MDA-MB-231 cells were cultured alone or together with WT mouse BM cells in the presence of M-CSF +/− PBS (P) or IL-4 for 3 d, during which 3 nM SM-164 and 1 μg/ml TNFR:Fc (R:Fc) were added for the last 16 hr (last group on right +R:Fc). Annexin V + PI +/− apoptotic cells in the GFP + population were analyzed by flow cytometry (left panel). The total number of GFP + cells (left graph) was calculated based on % of GFP + cells in the total cell number, and % of Annexin V + PI +/− apoptotic cells in the GFP + population (right graph) was calculated. *p < 0.05 and **p < 0.01, one-way ANOVA +/Dunnett test.
100 Ng/Ml Gmp Compliant Recombinant Human M Csf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/100 ng/ml gmp-compliant recombinant human m-csf/product/R&D Systems
Average 90 stars, based on 1 article reviews
100 ng/ml gmp-compliant recombinant human m-csf - by Bioz Stars, 2026-02
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Image Search Results


SM-164 induces apoptosis of breast cancer cells in combination with TNFα released by tumor-associated macrophages. ( A ) The parental MDA-MB-231 cells were treated with vehicle, TNFα (1 ng/ml) or TNFα + the indicated doses of SM-164 or BV6 overnight. Annexin V + PI +/− apoptotic cells were analyzed by flow cytometry. ( B ) Parental MDA-MB-231 cells were treated with the indicated doses of SM-164, BV6 or AT-406 alone, or with a combination of them plus TNFα (1 ng/ml) for 8 hours. Cell lysates were used to test protein levels of cIAP1/cIAP2 and GAPDH. ( C ) 1 × 10 4 GFP + MDA-MB-231 cells were cultured alone or together with WT mouse BM cells in the presence of M-CSF +/− PBS (P) or IL-4 for 3 d, during which 3 nM SM-164 and 1 μg/ml TNFR:Fc (R:Fc) were added for the last 16 hr (last group on right +R:Fc). Annexin V + PI +/− apoptotic cells in the GFP + population were analyzed by flow cytometry (left panel). The total number of GFP + cells (left graph) was calculated based on % of GFP + cells in the total cell number, and % of Annexin V + PI +/− apoptotic cells in the GFP + population (right graph) was calculated. *p < 0.05 and **p < 0.01, one-way ANOVA +/Dunnett test.

Journal: Scientific Reports

Article Title: The IAP Antagonist SM-164 Eliminates Triple-Negative Breast Cancer Metastasis to Bone and Lung in Mice

doi: 10.1038/s41598-020-64018-z

Figure Lengend Snippet: SM-164 induces apoptosis of breast cancer cells in combination with TNFα released by tumor-associated macrophages. ( A ) The parental MDA-MB-231 cells were treated with vehicle, TNFα (1 ng/ml) or TNFα + the indicated doses of SM-164 or BV6 overnight. Annexin V + PI +/− apoptotic cells were analyzed by flow cytometry. ( B ) Parental MDA-MB-231 cells were treated with the indicated doses of SM-164, BV6 or AT-406 alone, or with a combination of them plus TNFα (1 ng/ml) for 8 hours. Cell lysates were used to test protein levels of cIAP1/cIAP2 and GAPDH. ( C ) 1 × 10 4 GFP + MDA-MB-231 cells were cultured alone or together with WT mouse BM cells in the presence of M-CSF +/− PBS (P) or IL-4 for 3 d, during which 3 nM SM-164 and 1 μg/ml TNFR:Fc (R:Fc) were added for the last 16 hr (last group on right +R:Fc). Annexin V + PI +/− apoptotic cells in the GFP + population were analyzed by flow cytometry (left panel). The total number of GFP + cells (left graph) was calculated based on % of GFP + cells in the total cell number, and % of Annexin V + PI +/− apoptotic cells in the GFP + population (right graph) was calculated. *p < 0.05 and **p < 0.01, one-way ANOVA +/Dunnett test.

Article Snippet: MDA-MB-231 cells were treated with different doses of SM-164 +/− 1 ng/ml TNFα (R&D system Cat# 210-GMP) overnight.

Techniques: Flow Cytometry, Cell Culture

ADR-resistant (R) MDA-MB-231 cells are also resistant to SM-164, while SM-164-R MDA-MB-231 cells are sensitive to ADR. ( A ) Growth curve of the parental and ADR-R MDA-MB-231 cells, starting at 1 × 10 4 cells/well, in a well of 6-well-plates treated with 30 ng/ml ADR or vehicle, and passaged to 60- and 100-mm dishes, respectively, when untreated cells grew to sub-confluence. 3 wells/group, **p < 0.01, one-way ANOVA +/Dunnett test. ( B ) Parental and ADR-R MDA-MB-231cells were treated with the indicated doses of SM-164 plus 1 ng/ml of TNFα overnight. Annexin V + PI +/− apoptotic cells were analyzed by flow cytometry. ( C ) 0.5 × 10 4 parental and SM-R MDA-MB-231 cells in 60 mm dishes were cultured overnight followed by treatment with 1 ng/ml TNFα plus the indicated doses of SM-164 for 3 days, and surviving cells were counted. ( D ) The parental and SM-164-R cells (0.5 × 10 4 cells in 60 mm dishes) were treated with the indicated doses of ADR for 6 days and cell numbers were counted after digestion with 0.2% trypsin. **p < 0.01 vs. the respective parental cells, one-way ANOVA +/Dunnett test. The experiments were repeated 3 times with similar results.

Journal: Scientific Reports

Article Title: The IAP Antagonist SM-164 Eliminates Triple-Negative Breast Cancer Metastasis to Bone and Lung in Mice

doi: 10.1038/s41598-020-64018-z

Figure Lengend Snippet: ADR-resistant (R) MDA-MB-231 cells are also resistant to SM-164, while SM-164-R MDA-MB-231 cells are sensitive to ADR. ( A ) Growth curve of the parental and ADR-R MDA-MB-231 cells, starting at 1 × 10 4 cells/well, in a well of 6-well-plates treated with 30 ng/ml ADR or vehicle, and passaged to 60- and 100-mm dishes, respectively, when untreated cells grew to sub-confluence. 3 wells/group, **p < 0.01, one-way ANOVA +/Dunnett test. ( B ) Parental and ADR-R MDA-MB-231cells were treated with the indicated doses of SM-164 plus 1 ng/ml of TNFα overnight. Annexin V + PI +/− apoptotic cells were analyzed by flow cytometry. ( C ) 0.5 × 10 4 parental and SM-R MDA-MB-231 cells in 60 mm dishes were cultured overnight followed by treatment with 1 ng/ml TNFα plus the indicated doses of SM-164 for 3 days, and surviving cells were counted. ( D ) The parental and SM-164-R cells (0.5 × 10 4 cells in 60 mm dishes) were treated with the indicated doses of ADR for 6 days and cell numbers were counted after digestion with 0.2% trypsin. **p < 0.01 vs. the respective parental cells, one-way ANOVA +/Dunnett test. The experiments were repeated 3 times with similar results.

Article Snippet: MDA-MB-231 cells were treated with different doses of SM-164 +/− 1 ng/ml TNFα (R&D system Cat# 210-GMP) overnight.

Techniques: Flow Cytometry, Cell Culture